Protein_Domain

Part:BBa_K203101:Design

Designed by: Michael Bartoschek and Douaa Mugahid   Group: iGEM09_Heidelberg   (2009-10-15)

ER membrane targeting (C-terminal)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 133
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- This part was isolated from a plasmid provided as a curtsy of the [http://www.bioquant.uni-heidelberg.de/research/groups/screening_of_cellular_networks/home.html Starkuviene lab], BioQuant, Heidelberg, Germany.

- This part was designed to be fused C-terminally to the protein of interest and contains a stop codon but no start codon.

- This part is BBb compatible allowing it to be used directly for protein fusion.

-Mutagenesis PCR was necessary to remove a PstI site from the original sequence.

Source

Plasmid DNA

References